Fig. 6: The Beetroot fold supports heterodimer formation. | Nature Communications

Fig. 6: The Beetroot fold supports heterodimer formation.

From: Co-crystal structures of the fluorogenic aptamer Beetroot show that close homology may not predict similar RNA architecture

Fig. 6

a Schematic depiction of the heterodimer formed by one wild-type protomer fused to F30 scaffold23 and one Wobble Beetroot protomer. Asterisks denote secondary structure elements of the F30 scaffold. b Native polyacrylamide gel electrophoresis of the co-folded protomers and controls of F30 fused wild-type and Wobble homodimers. Dashed box indicates band excised for elution, red arrow indicates F30 fused wild-type homodimer, and mustard arrow indicates Wobble homodimer. Two independent experiments provided the same result. c Denaturing polyacrylamide gel electrophoresis of the eluted RNA from c and controls F30 fused wild-type and Wobble RNAs. Two independent experiments provided the same result. d Emission spectra of the heterodimer in the presence of DFAME only (red), DFHO only (mustard), and DFAME + DFHO (red–mustard) when excited at 470 nm.

Back to article page