Fig. 5: Defects in chromosome segregation induced by loss of Ser-384 RPA70 phosphorylation.

Representative immunofluorescent images stained with DAPI and anti-Tubulin antibody depict anaphase bridges (white arrow) in the mitotic population of asynchronous RPA^SA/SA mutant and RPA^WT/WT cells (a) and in cells released from prometaphase arrest (b). Images are representative of three independent experiments. Quantitation of anaphase bridges and lagging chromosomes in mitotic population of asynchronous cells (c) and in cells released from prometaphase arrest (d) RPA^WT/WT and RPA^SA/SA cells. More than 70 cells were counted from three independent experiments. Data represent SEM and statistical significance was determined using an unpaired two-tailed t-test. ***p = 0.0001 (c) and *** p = 0.0002 (d).