Fig. 5: Reduction of intracellular NOTCH4 (NICD4) sensitized EGFR-TKIs mediated by HES1. | Nature Communications

Fig. 5: Reduction of intracellular NOTCH4 (NICD4) sensitized EGFR-TKIs mediated by HES1.

From: NOTCH4ΔL12_16 sensitizes lung adenocarcinomas to EGFR-TKIs through transcriptional down-regulation of HES1

Fig. 5

a The diagram of potential downstream molecules of NOTCH4. b The relative mRNA expressions of potential downstream of NOTCH4. (n = 3 biologically independent experiments). c Western blot analysis of tumor cells in PDX/PDX-GR/PDX-OR and PC-9/ PC-9GR /PC-9OR cells. d The relative mRNA expressions in PC-9GR/PC-9OR with siNOTCH4 treatment. (n = 6 biologically independent experiments). e Western blot analysis of PC-9GR/PC-9OR with siNOTCH4 treatment. f and g The cell viability after siNOTCH4 with or without OE-HES1 treatment in PC-9GR/PC-9OR. (n = 3 biologically independent experiments). h and j The tumor growth curve of CDX models after siNOTCH4 with or without OE-HES1 treatments. (n = 5 CDXs). i and k The tumor weight of CDX models after siNOTCH4 with or without OE-HES1 treatments. (n = 5 CDXs) mean ± SD. An unpaired two-sided student t-test was performed in Fig. 5b, d, and 5f–k. Three biologically independent experiments were performed for Fig. 5c, e. Source data are provided as a Source Data file.

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