Fig. 6: 14-3-3ɣ binding to PEAK3tandem peptide reduces the affinity of CrkIINSH3 binding to the adjacent CrkII motif. | Nature Communications

Fig. 6: 14-3-3ɣ binding to PEAK3tandem peptide reduces the affinity of CrkIINSH3 binding to the adjacent CrkII motif.

From: Structural mapping of PEAK pseudokinase interactions identifies 14-3-3 as a molecular switch for PEAK3 signaling

Fig. 6

a Sequential ITC binding studies using purified 14-3-3ɣ and purified CrkIINSH3 domain and PEAK3tandem-S69 non-phosphorylated and b PEAK3tandem-pS69 phosphorylated peptides. b High affinity binding of 14-3-3ɣ to PEAK3tandem-pS69 peptide reduces the affinity of CrkIINSH3 binding to the adjacent CrkII motif at the tandem site (negative cooperativity). See Supplementary Fig. 6a–d and Supplementary Data 5 for all ITC data.

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