Fig. 7: S69A mutant increases PEAK3 p-Tyr and cell motility. | Nature Communications

Fig. 7: S69A mutant increases PEAK3 p-Tyr and cell motility.

From: Structural mapping of PEAK pseudokinase interactions identifies 14-3-3 as a molecular switch for PEAK3 signaling

Fig. 7

a S69A mutant increases PEAK3 p-Tyr. MCF-10A expressing WT and S69A PEAK3 cells were starved in –EGF medium overnight. Anti-HA IPs were prepared from denatured cell lysates and Western blotted as indicated. Data represent the mean ± S.E.M. of n = 3 independent experiments. **p < 0.01 (p = 0.0078), by ratio paired t-test, two tailed (Repeats shown in source data). b Effect of S69A PEAK3 on random cell motility in MCF-10A cells. Vector, HA-tagged WT or S69A PEAK3 were stably overexpressed in MCF-10A cells and random cell motility was determined by live cell tracking. Representative images are shown. Scale bar, 200 μm. The histogram indicates the average maximum displacement distance from the origin. ~40 cells were analyzed in each experiment. Data represent the mean ± S.E.M. of N = 3 independent experiments. ****p < 0.0001 by one-way ANOVA with Dunnett’s multiple comparisons test (Vector vs. WT, p = 0.00090; WT vs. S69A, p = 0.000003), (Repeats shown in source data). Source data are provided as a Source Data File.

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