Fig. 5: Multiplexing the BacCam workflow using multiple colors of light.
From: A biological camera that captures and stores images directly into DNA
A Design of a co-culture of engineered E. coli with blue and red light sensitive recombinases respectively. The blue light Opto-Cre-Vvd system and the redesigned red light recombinase recording system were separately transformed into E. coli. B Experimental workflow of the co-culture. Both resulting strains of E. coli were co-cultured together on the same plate, and exposed to either alternating blue and red light projections, or simultaneous projections of both blue and red light. C Images obtained after sequencing and deconvolution, separating red and blue with the red light specific barcode and subsequently subjecting it to our deconvolution algorithm workflow based on 3-component Gaussian Mixture Model (GMM). Source data are provided as a Source Data file.
