Fig. 2: Association of 21-nt U-phasiRNAs with AGO1b and AGO1d.
From: Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway
a Wes analysis of AGO1b and AGO1d proteins in developing anthers. AGO1b and AGO1d total proteins, extracted from stage 1 (ST1), stage 2 (ST2), stage 3 (ST3), stage 4 (ST4), stage 5 (ST5), and stage 6 (ST6) anthers, were enriched during meiosis and post-meiosis (stages 2–5). ST1 is pre-meiosis, or primordial germ cell initiation; ST2–4 are during meiosis; ST5 is the microspore development stage; and ST6 is the bicellular pollen stage. The wes using ST1, ST2, and ST3 total proteins was repeated with similar results. b Wes images of AGO1b/d RIP fractions with anti-AGO1b or -AGO1d. Arrowheads show the size distribution of AGO1b protein (magenta) and AGO1d protein (blue). The RIP analysis was performed three times with similar results. c Size distribution of AGO1b–small RNAs and AGO1d–small RNAs. d Relative frequency of each nucleotide in the AGO1b/d–small RNAs. e Pie charts summarizing the source of AGO1b/d–small RNAs. AGO1b and AGO1d mainly associate with 21-nt U-phasiRNAs derived from 21PHASs/lncRNAs. f Read counts of miR2118 family members in AGO1b–small RNAs and AGO1d–small RNAs. The reads data show the average counts of two replicates for AGO1b–small RNAs and four replicates for AGO1d–small RNAs.
