Fig. 1: Generation of the real-time cell proliferation reporter system.
From: A highly sensitive strategy for monitoring real-time proliferation of targeted cell types in vivo
A Construct of the real-time cell proliferation reporter system. The CAT/polyA element flanked by loxP sites (LSL cassette) was located downstream from the human Ki67 promoter (Ki67p) in the pGLuc Basic-1 vector and comprised the Ki67p-LSL-Gluc construct. After Cre recombination, the LSL cassette was derived and Gluc was expressed under Ki67p activity. B, C Luciferase activity in culture media of LacZ- or Cre-adenovirus infected Hepa1-6 cells B or MIN6 C cells relative to that in culture media of Cre-adenovirus infected Hepa1-6 cells (Cre-infected Hepa1-6). D–F Luciferase activity in culture media of LacZ- or Cre-adenovirus infected Hepa1-6 cells, after treatment with Mitomycin C D, Rapamycin E, or Roscovitine F relative to those in culture media of Cre-adenovirus infected cells treated with vehicle. Data are presented as means ± SEM. **p < 0.01, assessed by two-sided unpaired t-test B, C, or one-way ANOVA followed by Bonferroni’s post hoc test D–F. B, C n = 5 independent samples for each group. D–F n = 4 independent samples for each group. Results are representative of two independent experiments. Exact P values are B P = 2.9E-8; C P = 5.0E-8; D P = 2.6E-19 (LacZ + Vehicle vs. Cre + Vehicle), P = 2.1E-18 (Cre + Vehicle vs. Cre + Mitomycin C); E P = 4.1E-13 (LacZ + Vehicle vs. Cre + Vehicle), P = 5.6E-13 (Cre + Vehicle vs. Cre + Rapamycin); F P = 2.2E-24 (LacZ + Vehicle vs. Cre + Vehicle), P = 2.6E-24 (Cre + Vehicle vs. Cre + Roscovitine). Source data are provided as a Source Data file.
