Fig. 2: In vivo real-time monitoring of hepatocyte proliferation after partial hepatectomy.
From: A highly sensitive strategy for monitoring real-time proliferation of targeted cell types in vivo
A Time courses of luciferase activity in plasma of 10 weeks old male iLKi67p-Gluc mice on C57BL/6 background after partial hepatectomy (PHx, red) relative to those on day 0. 10 weeks old male iLKi67p-Gluc mice undergoing sham operation (SO, blue) served as controls. Solid lines and dotted lines indicate average and individual values, respectively. B Ki67-positive hepatocyte ratios in 10 weeks old male iLKi67p-Gluc mice on day 0, 2, or 9 after PHx; representative images are shown in the right three panels. Scale bars denote 50 µm. C Linear relationship between relative luciferase activity and Ki67-positive hepatocyte ratios. Open circles indicate relative luciferase activity and Ki67-positive hepatocytes in individual 10 weeks old male iLKi67p-Gluc mice after PHx relative to those on day 0 (r = 0.904, P = 5.3E-5). D In vivo bioluminescence imaging of 10 weeks old male iLKi67p-Gluc mice on day 3 after PHx (Cre (+) PHx). Ten weeks old male Ki67p-LSL-Gluc mice without the Cre-ER transgene which had undergone PHx (Cre (-) PHx), and iLKi67p-Gluc mice which had undergone sham operation (Cre (+) SO) served as controls. Representative images are shown. (E) Quantification of signal intensity in the PHx group on 0, 2, and 9 days after PHx; representative bioluminescence images are shown. Rectangles indicate regions of interest. Solid lines and dotted lines indicate average and individual values, respectively.Data are presented as means ± SEM. **p < 0.01, assessed by one-way repeated-measures ANOVA followed by the Tukey multiple comparison test for the various time points A, E, or one-way ANOVA followed by Bonferroni’s post hoc test B. Pearson’s correlation coefficient (two-sided) was used to determine the correlation C. A n = 4 independent animals for each group, from two independent experiments. B, C n = 4 independent animals for each group, from three independent experiments. (E) n = 4 independent animals, from three independent experiments. Exact P values are A P = 0.0027; B P = 0.0043 (day 0 vs. day 2), P = 0.0065 (day 2 vs. day 9); E P = 4.5E-4 (day 0 vs. day 2). Source data are provided as a Source Data file.
