Fig. 6: In vivo real-time monitoring of β-cell proliferation in the juvenile stage.
From: A highly sensitive strategy for monitoring real-time proliferation of targeted cell types in vivo
A Time courses of luciferase activity in plasma from male cβKi67p-Gluc mice on C57BL/6 background relative to those on week 12. Solid lines and dotted lines indicate average and individual values, respectively. B The diurnal variation in luciferase activity in plasma from 4 to 5 weeks-old male cβKi67p-Gluc mice. Luciferase activity relative to that on ZT 8 is shown. Solid lines and dotted lines indicate average and individual values, respectively. C The area under the curve of the plasma luciferase activity of 4 weeks-old male cβKi67p-Gluc mice during the dark (ZT12-24) or light period (0–12). Data are presented as means ± SEM. *p < 0.05, **p < 0.01, assessed by one-way repeated-measures ANOVA followed by the Tukey multiple comparison test for the various time points A, B, or two-sided paired t-test C. A n = 6 independent samples. Results are representative of two independent experiments. B, C n = 6 independent samples from two independent experiments. Exact P values are A, P = 9.4E-8 (4 weeks vs. 12 weeks), P = 1.7E-8 (5 weeks vs. 12 weeks), P = 0.0139 (6 weeks vs. 12 weeks); B P = 0.0086 (ZT 12 vs. ZT 20), P = 3.3E-4 (ZT 12 vs. ZT 0), P = 9.5E-5 (ZT 12 vs. ZT 4), P = 4.0E-4 (ZT 16 vs. ZT 0), P = 1.1E-4 (ZT 16 vs. ZT 4); C P = 0.021. Source data are provided as a Source Data file.
