Fig. 7: Ex vivo monitoring of β-cell proliferation using isolated islets from iβKi67p-Gluc mice.
From: A highly sensitive strategy for monitoring real-time proliferation of targeted cell types in vivo
A Luciferase activity in culture media of isolated islets from 3 months old male iβKi67p-Gluc mice on C57BL/6 background after stimulation with glucagon-like peptide-1 (GLP-1) for 72 h (72-h GLP-1). B Gene expression of mouse Ki67 in these islets. C Luciferase activity in culture media of isolated islets from 3 months old male iβKi67p-Gluc mice after stimulation with GLP-1 for 96 hours (96-h GLP-1). D Gene expression of mouse Ki67 in these islets. Luciferase activity in culture media of isolated islets cultured with vehicle or gene expression in these islets served as control (Vehicle).Data are presented as means ± SEM. n.s., not significant, *p < 0.05, **p < 0.01, assessed by two-sided unpaired t-test. A, B n = 7 independent samples for 72-h GLP-1, n = 6 independent samples for Vehicle. C, D n = 7 independent samples for each group. Exact P values are A, P = 9.4E-8 (4 weeks vs. 12 weeks), P = 1.7E-8 (5 weeks vs. 12 weeks), P = 0.00029; B P = 0.0017; C P = 0.0028. Source data are provided as a Source Data file.
