Fig. 1: Transcriptional landscape of meningeal NK cells and ILC1.

a Schematic overview of sorted meningeal or splenic CD3⁻/NK1.1⁺ cells. C57BL/6 mice (n = 50) were intracardially perfused with PBS, and CD3⁻/NK1.1⁺ cells were sorted from meninges or spleen. Figure created with BioRender.com. b UMAP representation of CD3⁻/NK1.1⁺ cells derived from meninges (3392 cells). Profiles colored are from clusters identified subsets of NK cells and ILC1. c Percentage of NK cell subsets and ILC1 obtained in the meninges and spleen. d Violin plots represent the distribution of ILC1 and NK cell gene expression programs defined in Robinette et al.⁷⁴, grouped by clusters in the meninges. e NK cells and ILC1 frequency in the meninges of C57BL/6 mice (n = 6). Right: representative FACS analysis of meningeal ILC1/NK cells. For boxplots, the center line, boxes and whiskers represent the median, inner quartiles, and rest of the data distribution, respectively. f DotPlot represent the expression levels of ILCs archetypal genes within the identified NK cells and ILC1 clusters in meninges and spleen. g Violin plots represent the distribution of indicated genes grouped by clusters and divided for tissue. h Heatmap of the scaled regulon activity for select regulons expressed in each NK cell and ILC1 population.