Fig. 4: Reduced GCLC expression and de novo glutathione synthesis in pancreatic cancer exposed to a relative high glucose state.
From: Increased glucose availability sensitizes pancreatic cancer to chemotherapy
a, b Significantly altered genes associated with redox metabolism (a) and qPCR analysis of glutathione metabolism-associated enzymes (b) in KPC orthotopic tumors under the indicated conditions (n = 5 orthotopic tumors per group). c, Immunolabeling of GCLC in independent KPC orthotopic tumors receiving D30 water versus control (representative immunoblots of three tumors with similar results are shown). Scale bars, 50 µm. d–g Relative GSH levels (d, n = 6 tumors per group; f, n = 5 tumors per group) and GSH/GSSG ratio (e, n = 6 tumors per group; g, n = 5 tumors per group) in indicated tumors. qPCR analysis in PDAC cells under the indicated conditions for 48 hours (h–j) (n = 3 independent experiments). k qPCR analysis of GCLC transcripts in murine orthotopic pancreatic tumors compared to normal pancreas (n = 5 per group). l, Box plot showing GCLC transcripts (TPM: transcripts per million) in human pancreatic tumors versus normal pancreas. For this gitter box plot, the center line indicates the median, box limits represent the upper and lower quartiles, and whiskers indicate the 1.5x interquartile range. These data were taken from TCGA and GTEx databases for tumor and normal pancreas, respectively, and were analyzed using GEPIA. The number of cases is indicated (P = 3.68e−13). Data are provided as mean ± s.d. (h–j) or mean ± s.e.m (b, d–g, k). Pairwise comparisons were conducted using two-tailed, unpaired Student’s t-tests. Source data are provided as a Source Data file.
