Fig. 4: Signaling via Lpar5 modulates antigen-specific killing in vivo. | Nature Communications

Fig. 4: Signaling via Lpar5 modulates antigen-specific killing in vivo.

From: Lysophosphatidic acid modulates CD8 T cell immunosurveillance and metabolism to impair anti-tumor immunity

Fig. 4

A Schematic of in vivo killing assay. B, C Representative flow cytometric histograms and dot plots of target cell input (left) and killing of target cells (right) pulsed with (B) N4 ovalbumin peptide or (C) HSV1 irrelevant peptide. D Frequency of ovalbumin-specific (tetramer+) CD8 T cells from the spleens of wildtype C57BL/6 mice and Lpar5−/− mice immunized with N4 ovalbumin peptide 4 days earlier where n = 3 mice per group and p = 0.8065. E Quantitative analysis of percent specific in vivo killing 5 days after ovalbumin peptide immunization and 1 day after transfer of pulsed target cells from panels A and B where n = 3 mice per group and p = 0.0422. F Schematic of adoptive transfer for in vivo killing assay. G Representative flow cytometric dot plots target cell killing pulsed with N4 ovalbumin peptide. H Quantitative analysis of percent specific in vivo killing 4 days after ovalbumin peptide immunization and 2 h after transfer of pulsed target cells where n = 3 mice per group and p = 0.0123. Statistics for this entire figure were performed using the unpaired two-sided Student’s t-test analysis was performed where *p < 0.05. Error bars for panels (D, E, H) represent standard error of the mean.

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