Fig. 6: Epigenetic regulation of ATE-G isoforms in the RPP4 locus and the impact on environmental responses and RNA stability.

a RPP4-ATCOPIA4 locus. TEs, Araport11 gene annotation, DRS-AtRTD3 transcript isoforms, and primers for RT-qPCR are shown. b Relative expression of RPP4 transcripts detected by RT-qPCR with primers indicated in a. Bars represent the means of four biological replicates ± SEM. *, p < 0.05 by t-test. c Relative transcript levels of RPP4 at 0, 30, 60, 90, and 120 min after cordycepin treatment in Col-0, ibm2, edm2, and suvh456. Expression levels at 0 min are set as 1. Bars represent the means of four biological replicates ± SEM. *, p < 0.05 by t-test. d Incompatibility of A. thaliana ecotypes and mutants against Hyaloperonospora arabidopsidis infection. NFA-10 and Kas-2 are ecotypes without the RPP4 locus and were used as controls. Class I (white), hypersensitive response surrounding conidia penetration sites; class II (light green), presence of trailing necrosis in ≤50% leaf area; class III (dark green), presence of trailing necrosis in ≤75% leaf area; class IV (black), compromised ETI immunity, presence of pathogen hyphae not targeted by HR and conidiophores. Statistically significant differences in the frequency distribution of the classes between lines and Col-0 were determined by Pearson’s chi-squared test; *, p < 0.05; **, p < 0.01; ***, p < 0.001. 70–130 leaves were analyzed per line across three separate experimental replicates. Source data are provided as a Source Data file.