Fig. 2: DAAO-TG^Cdh5 transgene expression in blood vessels and DRG neurons.

a shows representative immunofluorescence images of arterial preparations (from aorta or middle cerebral artery, as noted) that were isolated from untreated DAAO-TG^Cdh5 mice, and then fixed and stained as indicated with antibodies directed against VE-cadherin (endothelial cell marker) or GFP (which detects the YFP contained in the HyPer-DAAO fusion protein transgene). The scale bars indicate 100 μm. b shows a pictorial representation of the dorsal root ganglia (DRG) located adjacent to the lumbar spinal cord in the mouse. c shows representative immunofluorescence images of DRG isolated from untreated DAAO-TG^Cdh5 mice and stained with antibodies directed against the neuronal marker NeuN⁶⁷ or with GFP antibodies to detect the transgene, as indicated. The scale bars note 100 μm. d shows a photomicrograph of silver-stained fixed DRG that were isolated from D-alanine-treated DAAO-TG^Cdh5 mice; the dark staining is indicative of neuronal death⁶⁸ (The right-side figure is at a higher magnification of the inset in the left-side figure). The scale bars indicate 100 µm. e shows electron microscopic images of lumbar DRG isolated from D-alanine-treated TG^Cdh5 mice, revealing swollen and distorted mitochondria. The scale bars indicate 500 nm. f shows electron microscope images of lumbar DRG isolated from D-alanine-treated control mice. The scale bars indicate 500 nm. All images are representative of n = 3 animals per group.