Fig. 2: Performance of the cell-free antibody fragment screening workflow evaluated on SARS-CoV-2 neutralizing antibodies.
From: A rapid cell-free expression and screening platform for antibody discovery
a, b Heatmap of the binding of previously published antibodies measured using AlphaLISA to detect S6P binding (log10 scaled), RBD binding (log10 scaled), and ACE2 competition (linearly scaled). The lowest reported neutralization IC50 value is also plotted for comparison (log10 scaled) and an X indicates no relevant data available (Supplementary Data 1). a Heatmap of the binding of sdFabs derived from diverse sources. b Heatmap of the binding of sdFabs in the Brouwer et al. data set. c–e Parity plots comparing AlphaLISA measurements of the Brouwer et al. sdFabs vs. the previously reported Brouwer et al. data. c S6P binding AlphaLISA vs. S6P binding ELISA. d RBD binding AlphaLISA vs RBD binding ELISA. e ACE2 competition AlphaLISA vs pseudovirus neutralization IC50. c–e Black data points represent data that are statistically significant (two-sided t-test FDR corrected p < 0.05) from background and have an average value >3 standard deviations away from background. Grey data were considered not significantly different from background. a–e AlphaLISA data are the mean of 3 independent replicates (n = 3) derived from independent CFPS reactions. Antigen concentrations for AlphaLISA experiments listed in Supplementary Table 2. Source data are provided in the Source data file.
