Fig. 2: Large scale OXPHOS-related gene transcriptional upregulation and mitochondrial cristae biogenesis occur during flight muscle development.

a Transmission electron microscopy micrographs of wild-type flight muscle development spanning from wing-disc associated myoblasts to 2 days-old adult DLM. For each time point, a larger view (left, scale bar of 500 nm) and a zoom on mitochondria cristae (right, scale bar of 200 nm) are represented. The quantification of the number of mitochondrial cristae per length unit (500 nm) is shown for each corresponding time point except for 34 h APF. Mean and standard deviation are shown with each point representing a value of a single mitochondrion (n (Myo) = 12, n (48 h) = 19, n (64 h) = 15, n (72 h) = 23, n (pharate) = 21 and n (adult) = 47 mitochondria). For statistical analysis, one-way analysis of variance (ANOVA) was performed, followed by Tukey’s test for multiple mean comparisons (p values: Myo vs 48 h = 0.2; 48 h vs 64 h = 0 ****; 64 h vs 72 h = 0 ****; 72 h vs pharate = 0 ****; pharate vs adult = 0.002 **). b, c Transcriptional profile during flight muscle development of genes encoding structural subunits (b) and assembly factors (c) of the mitochondrial respiratory chain complexes. The solid lines represent the normalised mean of sequenced counts of all subunits/assembly factors of a given respiratory complex with shaded areas representing the standard error of the mean. Source data are provided as a Source data file.