Fig. 2: CFP1 epigenetically regulates uterine transcriptome via H3K4me3 on Day 4.

a Tag density of CFP1 binding and H3K4me3 peaks were calculated on ±3 Kbp window centered on TTS and CGI regions of all RefSeq (mm10) genes in Cfp1^f/f and/or Cfp1^d/d mouse uteri on Day 4 for heatmap and graph data. b CFP1-binding sequence logo of the top 10 motifs identified using de novo motif discovery. c Distribution of the genetic features across the mouse genome and CFP1-binding peaks in Cfp1^f/f mouse uterus on Day 4. d Correlation between H3K4me3 promoter (TSS ± 2 Kbp) enrichment conditions and gene expression in the mouse uterus on Day 4 (Cfp1^d/d versus Cfp1^f/f). Each dot represents a differentially expressed gene with statistical significance (p < 0.05, normalized data average, log₂ > 3). e Volcano plot to compare expression profiles from the Cfp1^f/f vs. Cfp1^d/d in the uterus on Day 4. f Pie chart summarizing upregulated or GO term in Cfp1^f/f versus Cfp1^d/d mouse uterus on Day 4. g GSEA to identify downregulated GO term and curated gene sets in Cfp1^f/f versus Cfp1^d/d mouse uterus on Day 4. Gene sets with an FDR q-value of <0.25 (red dotted line) were considered significant. h GSEA enrichment plot and heatmap of the “GO Regulation of smoothened signaling” gene set from RNA-seq data of Cfp1^f/f and Cfp1^d/d mouse uterus on Day 4. The color spectrum from blue to red indicates low to high expression. i RT-PCR and Real-time RT-PCR analyses for IHH-dependent SSP genes (red colored in h). n = 8 biologically independent samples per genotype. Data are presented as mean values with SD. Statistical analyses were performed using the unpaired Student’s t-tests. **p < 0.01.