Fig. 7: Knockdown of SART3 in NT2/D1 cells highlights potential interaction between SART3, NOVA2 and gonadal pathway genes. | Nature Communications

Fig. 7: Knockdown of SART3 in NT2/D1 cells highlights potential interaction between SART3, NOVA2 and gonadal pathway genes.

From: Variants in SART3 cause a spliceosomopathy characterised by failure of testis development and neuronal defects

Fig. 7

a–d shRNA mediated KD of SART3 in GFP-sorted NT2/D1 cells. a Mid and high GFP cells had 43 and 68% KD of SART3 mRNA respectively compared to non-GFP control cells, as determined by RT-qPCR. b NOVA2 mRNA levels correlated with SART3 KD. c FGF9 levels were also reduced, and d GATA4 expression was higher in KD cells. e SOX9 and f NR5A1 expression were not significantly different in KD cells. Expression is relative to the non-GFP control and shown as mean ± SEM. n = 4 independent experiments. A one-way ANOVA with Tukey’s multiple comparison test was used. P values are *<0.05; **<0.01; ***<0.001.

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