Fig. 2: Quantitative analysis of the S-nitrosoproteome in wild type and hot5-4.

a Volcano plot of S-nitrosylation sites identify in wild type and hot5-4 mutant. Red dots (n = 408) represent S-nitrosylated peptides enriched in hot5-4, (fold change > 1.5, p < 0.05, two-tailed unpaired t test). Blue dots (n = 71) represent S-nitrosylated peptides enriched in the wild type (fold change < 0.67, p < 0.05, two-tailed unpaired t test). b Analysis of S-nitrosylation of RPN12a, PSB33 and SDIRIP1 proteins in wild type and hot5-4 seedlings by in vivo biotin-switch method. The blots were detected by the anti-RPN12a, anti-PSB33 and anti-SDIRIP1 antibodies, respectively. Images shown are representative of at least three independent experiments. c Motif analysis of 408 S-nitrosylation sites enriched in hot5-4 mutant. Overrepresentation motifs of Cys-SNO sequence windows (−6 to +6 residues) constructed by pLogo. d S-nitrosylation motif analysis of 379 none up-regulated S-nitrosylation sites (0.75 <fold change < 1.2) in hot5-4 mutant. Overrepresentation motifs of Cys-SNO sequence windows (−6 to +6 residues) constructed by pLogo. e Biological process and molecular function distribution of 360 up-regulated S-nitrosylated proteins (p-value, Fisher’s exact test). f Enriched KEGG pathway in hot5-4 accumulated S-nitrosylated proteins.