Fig. 6: EPRS1 interacts with SARS-CoV-2 RNAs in a replicon system.
From: A viral pan-end RNA element and host complex define a SARS-CoV-2 regulon
a Schematic of SARS-CoV-2 ΔN-EGFP replicon assembled in a hybrid BAC (bacterial artificial chromosome)/YAC (yeast artificial chromosome) system (top). b Viral gene expression (EGFP) in 293 T cells transfected with replicon. c EPRS1 immunoprecipitation (IP) from flow-sorted GFP+ cells validated by immunoblot with anti-EPRS1 antibody. d RT-qPCR of viral RNAs from EPRS1-IP, expressed as fold-change compared to IgG-IP. ACTB; β-actin. Data are presented as mean values ± SD, n = 3 independent flow-sorting experiments; p values from two-tailed Student’s t test.
