Fig. 7: Antisense targeting of SARS-CoV-2 SPEAR element.
From: A viral pan-end RNA element and host complex define a SARS-CoV-2 regulon
a RNA-affinity pulldown of SPEAR-interacting EPRS1 from epididymal white adipose tissue (eWAT) from chow- and HFD-fed C57BL/6 J mice (top). Effect of HFD on body weight (center) and eWAT mass (bottom). Experiment was done once. b Schematics of AS RNAs (top-left) and morpholinos (Mph, bottom-left) targeting the SPEAR element. 5′-biotinylated SPEAR was used for RNA-affinity pulldown of EPRS1. AS SPEAR RNAs (top-right) were added before adding U937 extract, and morpholinos (bottom-right) were added during pulldown. Experiment was done twice. c Effect of morpholinos on 3′-end-SPEAR reporter (n = 4 independent biological replicates). d Dose-dependent inhibition of SPEAR-bearing sgRNA reporter expression (ns, p > 0.05; horizontal brackets, unpaired t tests; arrows, one-way ANOVA, n = 4 independent biological replicates). e Dose-dependent inhibition of spike plus interferon-γ-induced SPEAR sgRNA reporter expression by morpholinos in A549-hACE2 (n = 6 independent biological replicates) and Caco-2 (n = 4 independent biological replicates) cells (brackets and arrows as in d). Data are presented as mean values ± SD; p values from two-tailed Student’s t test.
