Fig. 2: Effects of the acla2 mutations on cellular acetyl-CoA levels and histone acetylation.
From: ACL and HAT1 form a nuclear module to acetylate histone H4K5 and promote cell proliferation
a Acetyl-CoA level assays in wild type (WT) and acla2 (acla2-1 and acla2-2) mutant seedlings (7 d after germination, DAG). Significant difference was calculated by the two-tailed, paired Student t test. Error bars represent means ± SD (n = 3) from three biological replicates. b Analysis of histone acetylation levels of acla2 (acla2-1 and acla2-2) and wild type (WT-1 and WT-2) seeds by immunoblotting using anti-histone acetylation antibodies. Seeds (at 6 d after fertilization, DAF) were used to extract histone proteins. Total histone extracts were used for the analysis. The H3 and H4 acetylation antibodies used for the tests are indicated on the left. Two replicates are shown. c Analysis of histone acetylation levels in wild type (two batches, WT1 and WT2) and acla2-1 and acla2-2 mutant seedlings (7 DAG) roots by immunoblotting using anti-histone acetylation antibodies. Total histone extracts were used for the analysis. Two replicates are shown. Immunoblotting bands were quantified using ImageJ and the relative signals indicated below each band were normalized with WT1 set as 1. Source data are provided as a Source Data file.
