Fig. 5: Validation of compiled positive hits from agonist-dependent HTS in dose-response. | Nature Communications

Fig. 5: Validation of compiled positive hits from agonist-dependent HTS in dose-response.

From: Profiling of basal and ligand-dependent GPCR activities by means of a polyvalent cell-based high-throughput platform

Fig. 5

a–o HTTL-B1, HTTL-B2 and HTTL-F cells were plated in cumate-containing (30 µg/mL) medium and transfected with potential GPCR hits identified from the agonist-dependent HTS. Transfected cells were stimulated with the receptor specific agonist and dose- response curves were built using XY analysis for non-linear regression curve and the 3-parameters dose-response stimulation function, followed by baseline correction. Data are presented as mean values, with error bars representing SEM. Data are representative of 2 biological replicates, with 3 technical replicates each. Generic receptor codes refer to the GPCR-Tango constructs.

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