Fig. 4: Cardiomyocyte-specific overexpression of full-length Tisp40 prevents I/R-induced acute cardiac injury, remodeling, and dysfunction in mice.

a Representative Evans blue and TTC-stained heart sections, and quantitative data from cardiomyocyte-restricted Tisp40 transgenic (cTG) mice and the matched non-transgenic (NTG) littermates 24 h after I/R surgery (n = 8). b Representative TUNEL staining images of heart sections and quantitative results from Tisp40 cTG mice and WT littermates 24 h after I/R surgery (n = 6). c DNA fragments in the heart (n = 6). d Caspase3 activity in the heart (n = 6). e Representative DHE staining images of heart sections and quantitative results from Tisp40 cTG mice and WT littermates 24 h after I/R surgery (n = 6). f Quantitative results of O₂⁻ and H₂O₂ in the heart (n = 6). g Heart samples were collected for WGA staining to quantify the cross-sectional area of cardiomyocytes 4 weeks post-I/R surgery (n = 6). h Heart samples were collected for PSR staining to quantify the collagen deposition 4 weeks post-I/R surgery (n = 6). i Levels of lysyl oxidase (Lox) mRNA in the heart 4 weeks post-I/R surgery (n = 6). j Total and insoluble collagen content in the heart 4 weeks post-I/R surgery (n = 6). k Cardiac function of Tisp40 cTG mice or NTG littermates was analyzed by transthoracic echocardiography at the indicated time points, and presented as FS, LVIDd and LVIDs (n = 6). All data are expressed as the mean ± SD, and analyzed using one-way ANOVA followed by Tukey post hoc test. For the analysis in (a, b, e), an unpaired two-tailed Student′s t test was conducted. For the analysis in (k), repeated measures ANOVA followed by Sidak post hoc test was conducted. *P < 0.0001. Source data are provided as a Source Data file.