Fig. 8: EpiHSP70s are key context-dependent regulators of mitosis in epiHSP70s-positive cancers. | Nature Communications

Fig. 8: EpiHSP70s are key context-dependent regulators of mitosis in epiHSP70s-positive cancers.

From: Systems-level analyses of protein-protein interaction network dysfunctions via epichaperomics identify cancer-specific mechanisms of stress adaptation

Fig. 8

a–d Localization and expression of epichaperomes in interphase and mitotic cells monitored by IF (a, b, d) and Native-PAGE (c). Micrographs are representative of n = 30 mitotic cells. epiTCO, epichaperome detection reagent; DAPI, chromosomes stain. Mitotic proteins and structures are shown for reference: NuMA (at spindle pole in mitotic cells and in the nucleus in interphase cells), pericentrin (PCNT, centrosome), α-tubulin (spindle). Scale bar, 5 μm. Graph (c), mean of n = 3 experiments. Graphs (d), median, dotted line and quartiles, dashed lines, one-way ANOVA, n = 40 cells, p < 0.0001, F (3, 156) = 537.7, with Sidak’s post-hoc. See also Supplementary Fig. 22. e, f Cell cycle (e) and confocal microscopy (f) analyses of epiHSP70s-high/medium (MDA-MB-468 and HeLa) and -low/negative (ASPC1) cells released from thymidine block into Vehicle or YK198 (2 µM). Graphs: (e) mean, n = 3 and (f) one-way ANOVA; n = 20 captured areas, p < 0.0001, F (3, 76) = 23.82 with Sidak’s post-hoc. See also Supplementary Figs. 23–26. g Graphs (mean) and micrographs (representative time-lapse microscopy images) of cancer cells released from thymidine block into Vehicle (n = 85 cells) or YK198 (n = 103 cells). Micrographs shows representative cells as they enter mitosis, fail to establish a proper mitotic plate, and undergo apoptosis. Scale bar, 25 μm. See also Supplementary Fig. 27. Source data, along with relevant statistical analyses and analysis data, are provided as a Source Data file.

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