Fig. 3: Copper effects on selenium homeostasis in LPP rats.
From: Excessive copper impairs intrahepatocyte trafficking and secretion of selenoprotein P
a Hepatic and (b, f) plasma Cu and (d, g) Se concentrations of female and male control rats (ATP7B+/−) and ATP7B−/− at different stages of Wilson’s disease (affected, disease onset, diseased) (n = 6–16) or (f–h) 8 days after application of the Cu chelator methanobactin (n = 3–4) were measured using TXRF with 1 mg/L yttrium as standard element for 300 s and normalized to protein content or with 1 mg/L gallium as standard element for 1000 s, respectively. c The CP oxidase (CPO) activity in plasma of rats was measured photometrically. e, h Plasma SELENOP was analyzed by Western Blot and normalized to Ponceau (P) staining (n = 5–8). Data are depicted as mean ± SD. Biological replicates are indicated by individual dots. Statistical analyses were based on one-way ANOVA with Bonferroni’s post-test *p < 0.05; **p < 0.01; ***p < 0.001 vs. ATP7B+/- control rats (a–e) or by two-tailed t test compared to untreated rats (f–h). Source data are provided as a source Data file.
