Fig. 2: npr1 mutants are hypersusceptible to TuMV infection.
From: A plant RNA virus inhibits NPR1 sumoylation and subverts NPR1-mediated plant immunity
a Phenotypes of WT plants and npr1 mutants agroinfiltrated with infiltration buffer (mock) or agrobacteria harboring TuMV-GFP under white light (WL) and ultraviolet light (UV) at 14 dpi. Red and green colors in the pseudocolor (PC) panel refer to virus-free and virus-infected areas, respectively. b Bar chart showing the ratios of TuMV-infected to the total leaf area of WT plants and npr1 mutants at 14 dpi (n = 5). c Bar chart of the relative levels of the viral genome in WT plants and npr1 mutants at 14 dpi. RT‒qPCR was performed with Actin II as the internal control, and the viral genome in the WT was normalized to 1 (n = 5). d Bar chart of the relative virion amounts in WT plants and npr1 mutants at 14 dpi. ELISA reads were taken after 1 h of substrate hydrolysis (n = 5). e Bar chart of the ratios of cytoplasmic NIb (NIbCyt) to nuclear NIb (NIbNuc) in WT or npr1 mutants (n = 5). f Bar chart of the relative PR1 expression level in mock- or TuMV-infected WT plants and npr1 mutants at 48 hpi (n = 3). RT‒qPCR was performed with Actin II as the internal control, and the PR1 level in mock-infected WT plants was normalized to 1. Data are presented as mean values ± SD. Statistical analyses were performed using Two-tailed Student’s t test. Source data are provided as a Source Data file.
