Fig. 5: Targeting SIM3 of NPR1 is a conserved function of NIbs from diverse potyviruses.
From: A plant RNA virus inhibits NPR1 sumoylation and subverts NPR1-mediated plant immunity
a, b Evaluation of interactions between NIb from different potyviruses and NPR1 (a) or sim3 (b) by Y2H. Due to the high-level of autoactivation of full-length NIb of SMV, only the C-terminal domain harboring SIM2 (SMVNIb-C) was used. c, d Evaluation of the interaction between potyviral NIb proteins and NPR1 (c) or sim3 (d) by BiFC. Micrographs were taken at 2 dpi. Scale bars = 50 μm. The experiment was independently repeated three times with similar results. e Influence of different potyviral NIb proteins on the luciferase activity of N. benthamiana leaf regions expressing cLUC-SUMO3 and NPR1-nLUC. All constructs were infiltrated at the same concentrations.
