Fig. 4: Mutant ARPC5 functional studies. | Nature Communications

Fig. 4: Mutant ARPC5 functional studies.

From: Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signaling

Fig. 4

a Fibroblasts from patient 1 (P1) and a healthy control (HC) were seeded on a retronectin-coated surface and fixed after 20 min. Actin protrusions were assessed via immunofluorescent staining with phalloidin. Adhesion and spreading of fibroblasts were monitored via real-time, impedance-based cell assay (graph). Impedance values are reported as cell index (CI). Curves represent the mean CI value with individual replicate data shown as dots. b Light microscopy images from P1 and HC neutrophils. The scale bars indicate 10 µm. The area over time covered by P1’s neutrophils compared to HC is shown in the graph. Curves represent the mean (±standard deviation) area from 5 to 6 cells. c Wound healing assay conducted with P1’s and HC’s fibroblasts. The gap length shown at 0 h corresponds to 0.94 mm. d Neutrophil chemotaxis in response to N-formylmethionyl-leucyl-phenylalanine (fMLF). The cells were incubated for 1 h and images were captured every 30 s. Single cell migration traces of ten randomly selected cells are shown. The position of each cell was anchored at the origin at t = 0. All results in this figure are representative of at least two independent experiments. Source data are provided as a Source Data file.

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