Fig. 4: NFIC regulates aspects of protein biosynthesis in the pancreas.

A IF analysis with an antibody recognizing 5.8 S rRNA and CDH1 shows decreased expression of both in Nfic^-/- acinar cells. (Scale bar = 5 μm). Quantification is shown in the accompanying panel (n ≥ 6/group, P < 0.05 (*), two-tailed Mann–Whitney U-test) (P = 0.038). B RT-qPCR analysis showing altered ribosomal RNA maturation in Nfic^-/- pancreata (n = 4/group; P < 0.01 (**), two-tailed Student T-test) (18 S, P = 0.01); 5.8 S Junction, P = 0.04; 5.8 S, P = 0.01; 28 S Junction, P = 0.01; 28 S, P = 0.002; 45 S, P = 0.003. C Boxplot plot displaying the relationship between the expression of upregulated, down-regulated, or a random set of genes, in control Nfic^+/+ vs. Nfic^-/- mice and in histologically normal human pancreatic tissues samples [top 10 low- vs. top 10 high- NFIC expressing samples, as determined by RNA-Seq analysis (NFIC^low vs. NFIC^high)]. Data shows the concordant pattern between down-regulated genes in Nfic^-/- mice and NFIC^low human pancreata. “N-1” two-tailed chi-squared test was used to calculate statistical significance. The P-value was calculated comparing to a random gene list. D GSEA for genes that are concurrently down-regulated in Nfic^-/- vs. WT pancreata and in NFIC^low vs. NFIC^high human pancreata. Genes were computed with KEGG data sets showing the similarities with those gene sets under-represented in Nfic^-/- mice. E Bar plot displaying the lower expression of genes coding for ribosomal proteins in NFIC^low vs. NFIC^high human pancreata (RPS5, P = 3.1 e-4; RPS8, P = 5.2 e-5; RPS11, P = 4.1 e-4; RPS15, P = 7.3 e-4; RPS21, P = 4.6 e-7; RPS26, P = 8.2 e-3; RPS29, P = 5.3 e-4; an integration of Fisher’s exact test and likelihood ratio were used to calculate statistical significance). Barplots are presented as mean values +/− SD. Boxplots show the minimum, the maximum, the sample median, and the first and third quartiles. Source data are provided as a Source Data file.