Fig. 3: Mice corneal alkali burn repair during the BPCL intervention.
a The anatomical detail of the injury cornea with the alkali burn area highlighted in red. b H&E staining images of the normal cornea and initial alkali burn cornea. c A series of images on the alkali burn cornea over time of the MN, MI, MS, and MB groups (n = 10) (Male: n = 1–5; Female: n = 6–10). d Corneal opacity score over time in different mouse groups. e Comparison of corneal opacity for different mouse groups on day 6. P = 0.03359 for MN and MI, P < 3.5 × 10-4 for MN/MI and MS/MB. f Corneal repair rate over time of the injury area. g Comparison of corneal repair rate on day 6 for different mouse groups P < 3 × 10−5 for MI and MS/MB. n = 10 independent mice in Fig. 3d–g. h H&E staining images of post-intervention corneas at the alkali burn site. i Comparison of epithelial thickness for different mouse groups, P < 3.5 × 10−8 for MN/MI and MS/MB, n = 10 independent samples. All data in (d) and (f) are presented as means ± SD. In box plots (e, g, i), the dot is the mean, the centerline is the median, box limits are the lower quartile (Q1) and upper quartile (Q3), and whiskers are the most extreme data points that are no more than 1.5 × (Q3 - Q1) from the box limits. Data were analyzed by non-parametric two-sided Mann–Whitney U test (e) and parametric two-tailed Student’s t-test (g, i). n.s, *, and *** represent nonsignificant (P > 0.05), P < 0.05 and P < 0.001, respectively. Differences were considered significant at P < 0.05. MN normal mice without cornea injury, MI mice in the intervention group, MS mice in the sham group, MB mice in the blank control group.
