Fig. 4: SIVETs enhance the relative levels of activated antigen-presenting cells in tumors.
a Schematic of the experiment. b Total numbers of tumor-infiltrating CD45+ cells per mm3 of tumor volume. P-value was determined by two-tailed one-way ANOVA with Geisser–Greenhouse correction. Pairwise P-values were determined by performing Tukey. Adjusted P-values < 0.05 are shown. Data are mean ± s.d. from n = 3 mice per condition for a single experiment. c Immunofluorescence imaging of myeloid cells expressing the indicated markers in NT, TcellOnly_Depot and SIVET_GMCSF tumors. Images are tiled acquisitions from a slide selected from 30 continuous sections each from 1 mouse per condition. d Umap plots showing the expression of the indicated markers. e Umap density plots of individual treatment conditions showing distinct localization of cells based on treatment group. Denser (hot) regions indicate more cells. f Umap plot overlaid with Kmeans clusters of immune cells in tumors. g Heatmap plot showing the average expression of the indicated immune cell markers in each cluster after K-means analysis. h Heatmap plot showing the proportion of cells in each condition represented in each cluster. Some clusters of interest are highlighted. i Scatterplot comparing the relative enrichment levels of the activated antigen-presenting cell population (cluster 2), and the poorly antigen-presenting cell population (cluster 6) as a function of the treatment group. Data are pooled cells from n = 3 mice per condition for a single experiment.
