Fig. 2: MAM-Calflux visualizes the changes in MAM-specific calcium dynamics.
From: Ratiometric measurement of MAM Ca2+ dynamics using a modified CalfluxVTN
a–e Time-lapse imaging of MAM-Calflux with serial treatments of 10 μM histamine and 10 μM ionomycin with 1 mM CaCl2. Representative images (a) and time-dependent plots for BRET ratio (b) and Venus excitation (d). Bar graphs represent the BRET ratio (c) and Venus excitation intensities (e) before and after histamine and CaCl2/ionomycin treatments. (n = 15 cells for serial treatment group). The scale bar represents 20 μm. All results are presented as means ± SEM for b, d and box plots representing median and interquartile range with whiskers min/max value and the cross representing mean value for c, e. All P values were calculated using two-tailed Student’s t test comparing before and after each treatment for c, e. Source data from b–e are provided as a Source Data file.
