Fig. 4: HOF Gating effect for enhancing the biocatalytic selectivity. | Nature Communications

Fig. 4: HOF Gating effect for enhancing the biocatalytic selectivity.

From: Green synthesis of stable hybrid biocatalyst using a hydrogen-bonded, π-π-stacking supramolecular assembly for electrochemical immunosensor

Fig. 4

a The cryo-EM image identifying the rhombus-shaped pore windows from the [10\(\bar{1}\)] projection, and the inset is the enlarged domain. The pyrene core and formic acid arm in HOF structure are shown as dark green prismatic square and purple ball, respectively. b The schematic illustration of the gating effect of Lipase@HOF-100 for ester hydrolysis. The color used in NPB and p-NPP models are: while ball for H atom; pink ball for C atom; blue ball for N atom; cyan ball for O atom. While the pyrene core, formic acid arm and Lipase in Lipase@HOF-100 structure are shown as dark green prismatic square, purple ball and yellow surface model, respectively. c Plot of reaction velocity, V, against substrate [NPB]. The dosages of for Lipase and Lipase@HOF-100 were kept at the same in each trial (80 µg/mL). The data of 3 independent experiments and calculated error bars (SD) are presented in c, SD = Standard Deviation (n = 3). Data are presented as mean values +/−SD. d The catalytic kinetics curves of free Lipase and Lipase@HOF-100 under 0.2 mM p-NPP. The dosages of Lipase (80 µg/mL) used were kept at the same in each trial.

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