Fig. 4: SEC-seq: single-cell transcriptomic sequencing associated with IgG secretion. | Nature Communications

Fig. 4: SEC-seq: single-cell transcriptomic sequencing associated with IgG secretion.

From: SEC-seq: association of molecular signatures with antibody secretion in thousands of single human plasma cells

Fig. 4

a Workflow for SEC-seq to link IgG secretion to transcriptome at the single-cell level. Partially created with BioRender.com. b Transcriptome-based clustering of single-cell expression profiles of human ASCs (right), and the levels of the corresponding levels of the anti-IgG barcode projected on the same UMAP plot (left; this data is compiled from 12,892 cells derived from three independent experiments and three donors). c Distribution of cells from each donor overlaid onto the UMAP plot. d The percentage breakdown of each donor’s cells represented in each cluster in the UMAP is shown. e Transcription factor gene expression levels for a marker of activated B cells (PAX5) and PCs (PRDM1, XBP1) overlaid on the UMAP plot and f illustration of canonical marker expression along the PC differentiation axis. All UMAP color bars are according to normalize expression data into units of log transcripts.

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