Fig. 5: IgG secretors are associated with different gene expression clusters and pseudotime.

a IgM, IgA, and IgG expressing populations are gated based on the mRNA expression level of IGHA and IGHM. Cells with IGHGx transcripts are highlighted in green. Each population was projected on the gene expression UMAP plot. b Percentage of the total cell population from each donor following in IgM, IgA, IgG, and activated B cell categories. c Histograms of unique IgG barcode reads in IgA/IgM (top) and IgG (middle, bottom) subsets. Dashed lines on the bottom histogram define the thresholds for SEC-IgG hi and SEC-IgG lo cells. d Percentage of all cells and IgG expressing cells that are SEC-IgG hi and SEC-IgG lo. e SEC-IgG hi cells (red) and SEC-IgG lo cells (blue) projected onto the UMAP; Activated B cells, IgG clusters, IgA/M clusters, and light chain clusters are highlighted on the UMAP. Bar graphs display the percentage cells in the three thresholded regions in (c) for each indicated cluster corresponding to the upper panel. f Single-cell graph by PAGA trajectory inference, and heatmap by inferred pseudotime of all cells. Trajectory starts from the initial state node (cluster 6, activated B cells from day 10 cells). Cell differentiation states in between are labeled according to their representative cell status. Heatmap vmin (minimum value to anchor the colormap) = 0.80. g The percentage of SEC-IgG hi cells in each 0.5 pseudotime frame (e.g., x-axis = 0.875 stands for 0.85–0.9 interval) for each donor is plotted as a function of pseudotime. The data is from 12,892 cells derived from three independent experiments and from three individual donors.