Fig. 6: Correlation analysis of SEC-seq data to find surrogate PC markers by IgG secretion ability. | Nature Communications

Fig. 6: Correlation analysis of SEC-seq data to find surrogate PC markers by IgG secretion ability.

From: SEC-seq: association of molecular signatures with antibody secretion in thousands of single human plasma cells

Fig. 6

a Schematic showing how we establish correlation scores between mRNA transcript levels and the SEC-IgG barcode. Created with BioRender.com. b Scatter plots showing donor-by-donor correlation scores between SEC-IgG and individual mRNA transcripts. The highest correlated genes across all donors are labeled. c Top ~40 highly consensus correlated genes sorted by mean of correlation coefficient from three donors. Heatmap represents the subgroup (SEC-IgG lo, SEC-IgG hi from all cells) mean of the z-score of each gene expression from each donor. Immunoglobulin are labeled in bold, known PC markers are labeled in red, and genes expressed on cell surface are labeled in purple. The data is from 12,892 cells derived from three independent experiments and from three individual donors. Source data are provided as a Source Data file.

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