Fig. 7: Analysis of SEC-seq data from IgG cells to identify enriched genes associated with IgG secretion. | Nature Communications

Fig. 7: Analysis of SEC-seq data from IgG cells to identify enriched genes associated with IgG secretion.

From: SEC-seq: association of molecular signatures with antibody secretion in thousands of single human plasma cells

Fig. 7

a Bar graphs display the percentage of cells in the three thresholded regions using thresholds of SEC-IgG lo and SEC-IgG hi from each sub-isotype of IgG cells. b Scatter plots of IGHG log transcripts and log IgG barcode reads (SEC-IgG). r is the Pearson correlation coefficient, and the p value was the result of a two-tailed t-test relative to the null hypothesis (no correlation). c Top differentially expressed genes of SEC-IgG hi. Heatmap represents the subgroup (SEC-IgG lo, SEC-IgG hi from IgG cells) mean of z-score of each gene expression from each donor. Source data are provided as a Source Data file. d Gene set enriched list in Hallmark and biological process from gene set enrichment analysis (GSEA), heatmap represents the normalized enrichment score (NES), FDR stands for false discovery rate. e Histograms of IgG secretion levels compared with high/low mitochondria activity, high/low ER amount, and 2-NBDG (glucose analog) uptake ±. f Schematic cartoon of genes upregulated in IgG secretors (adjust p values <0.1) based on different gene function clustering (DAVID) and GSEA. The data is from n = 8106 IgG cells derived from three independent experiments and from three individual donors. Created with BioRender.com.

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