Fig. 5: The NAD salvage pathway in mesenchymal cells is required for post-natal endochondral, but not intramembranous, bone formation.
From: The NAD salvage pathway in mesenchymal cells is indispensable for skeletal development in mice
a MicroCT images from femur of Namptf/+ (control) and Namptf/f;Prx1-Cre (NamptΔPrx1) mice at P28. b H&E staining performed on histological sections of knee, tibia, and ankle of NamptΔPrx1 mice to illustrate the lack of mineralized secondary ossification center (n = 3 mice/group). c Representative pictures of H&E staining of undemineralized histological sections of tibia (left) and quantification of osteoblast numbers at the endocortical surfaces (right) (n = 3 mice/group). Black arrows indicate osteoblasts covering the endocortical surfaces. BM=bone marrow. d Representative pictures of double calcein labels at the endocortical surfaces indicated by the white arrows (left) and quantification of mineral apposition rate (MAR) (right). Dotted white line marks the bone surface. e Representative pictures of male mice. f Whole body weight, and (g) DXA BMD of 1 mo-old male (n = 5 NamptΔOsx1 and 12 Osx1-cre mice/group) and female mice (n = 8 NamptΔOsx1 and 11 Osx1-cre mice/group). Lines represent mean ± SD, p values by two-sided Student t-test. Source data are provided as a Source Data file.
