Fig. 6: High content compound screening to find lipophagy activators.
From: Liver lipophagy ameliorates nonalcoholic steatohepatitis through extracellular lipid secretion
a Schematic diagram showing the image-based reporter system to quantify lipophagy activity level. GFP loses its fluorescence in autolysosomes resulting in a decrease in total fluorescent intensity of GFP in mCherry positive area. b The quality of lipophagy reporter system was confirmed by flow cytometry with LDTS-LIRs. c Automated imaging of lipophagy reporter cells expressing LDTS-LIRs as controls. Scale bars, 100 µm (n = 4 per group). d Robust Z score of approximately 3500 small molecules library. Toxic compounds defined as cell number <80% were excluded. e Individual validation of lipophagy and assessment of intracellular lipid lowering effect among 23 out of 39 primary hits, digoxin and other PI3K-mTOR inhibitors. All data are presented as mean ± SD. P values calculated by two-sided unpaired t-test. Source data are provided as a Source data file.
