Fig. 3: Phenotypic characterization of L- and S-strain.
From: Coexisting ecotypes in long-term evolution emerged from interacting trade-offs
a Single-strain growth rates in glucose minimal medium batch culture (upper panel, n = 4 biological replicates, unpaired t-test was performed, P-value = 0.044) and corresponding acetate excretion rates (lower panel, n = 4 biological replicates, unpaired t-test was performed, P-value = 0.0052). Error bars represent standard deviation. b Diauxic shift from glucose to acetate. Medium contains typical glucose concentration of LTEE (139μM) with additional acetate (10 mM). n = 2 replicate (technical) well for each strain used and mean values plotted, for details please refer to method. c Schematic illustration of the interplay of acetate excretion and lag times resulting in coexistence. The L-strain is a fermentation specialist, enabling faster growth on glucose via acetate excretion (left). The S-strain is specialized in switching to acetate due to its slower growth rate on glucose (right). Source data is provided as a source data file.
