Fig. 3: Intranasal immunized with dNS1-RBD induces TRMs cover respiratory tract. | Nature Communications

Fig. 3: Intranasal immunized with dNS1-RBD induces TRMs cover respiratory tract.

From: Intranasal influenza-vectored COVID-19 vaccine restrains the SARS-CoV-2 inflammatory response in hamsters

Fig. 3

A Representative flow cytometry contour plots for CD8a+ TRMs in NALT and lung at 30 days post last vaccination, control (gray), dNS1-Vector (blue) and dNS1-RBD (red). B, C Bar graph showing the frequency of CD44+ CD8a+ T cells (B) and CD8+ TRMs (C) in NALT and lung 30 days post a prime-boost vaccination. n = 6 biologically independent mice/group, control (gray), dNS1-Vector (blue) and dNS1-RBD (red). D, E Bar graph depicting the absolute number of CD44+ CD8a+ T cells (D) and CD8a+ TRMs (E) in the lungs at indicated time points after prime and booster immunization. n = 6 biologically independent mice/group, dNS1-Vector (blue) and dNS1-RBD (red). F, G Bar graph depicting the frequency (F) and the absolute number (G) of TRMs in the lungs after dNS1-RBD immunization (1 × 106 PFU/mL, 1 × 105 PFU/mL, 1 × 104 PFU/mL, 1 × 103 PFU/mL, 1 × 102 PFU/mL). n = 6 biologically independent mice/group, compared with the 1 × 106 PFU/mL group is represented in pink (1 × 105 PFU/mL in orange, 1 × 104 PFU/mL group in yellow, 1 × 103 PFU/mL in purple, and 1 × 102 PFU/mL in green). H Experimental design and schema, created with adobe illustrator 2020. I Bar graph depicting the numbers of IFN-γ SFCs from lung after stimulation of a peptide pool covering the entire spike protein. n = 7–10 biologically independent mice/group, group A: 2 doses of STFK (green), group B:2 doses of STFK and boost with dNS1-RBD (red), group C: single dose of dNS1-RBD (blue), group D: control (gray). J Bar graph depicting the frequency of TRMs in the lungs after booster immunization. n = 9–10 biologically independent mice/group, group A: 2 doses of STFK (green), group B:2 doses of STFK and boost with dNS1-RBD (red), group C: single dose of dNS1-RBD (blue), group D: control (gray). Data are presented as mean ± SEM. Statistics analysis were Mann–Whitney tests (two-sided). Source data are provided as a Source Data file. SFCs: spot-forming cells; TRMs: tissue-resident memory T cells; NALT: Nasal-associated lymphoid tissue.

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