Fig. 1: RNA-seq approaches.

Schematic of adapter ligation and RNA shearing for 5′RNA-seq³⁷, total RNA-seq (modified from the RNAtag-seq methodology⁴¹) and 3′RNA-seq (modified from the Term-seq methodology¹⁸). 5′ phosphates (circled ‘P’), RNA 5′ end adapter (green line) and RNA 3′ end adapter (red line) are indicated. 5′RNA-seq identifies and distinguishes transcription start sites (TSSs) from processed 5′ ends. Total RNA-seq captures sequencing read coverage across transcripts. 3′RNA-seq identifies RNA 3′ ends. Expanded figure in Supplementary Information (Fig. S1); modified from ref. ¹².