Fig. 1: Oncogenic truncated APC topology in CRC models. | Nature Communications

Fig. 1: Oncogenic truncated APC topology in CRC models.

From: Mutant APC reshapes Wnt signaling plasma membrane nanodomains by altering cholesterol levels via oncogenic β-catenin

Fig. 1

A Lollipop plot displaying the distribution and classes of mutations in the APC protein sequence across multiple bowel-associated CRC datasets in the cBioPortal for cancer genomics (https://www.cbioportal.org). Key mutations utilized throughout our studies are highlighted. The functional domains in the APC sequence are also highlighted and matched with their respective coding exons. cBioPortal was employed to create the illustrative depiction of the APC protein. B Summary of all the oncogenic truncated APC models utilized herein. C Characterization of truncated Apc gene products. Lysates were obtained from mouse (YAMC, IMCE, and IMCE βcat) and human (HCT116Δ, HCT116 SW480, DLD1, and HT29) cultured cells as well as patient-derived organoids (PDOs) and detected with a primary antibody against truncated APC. As controls, primary antibodies against βcat and housekeeping genes, β-actin and GAPDH, were utilized. Two independent western blots were performed two times displaying similar results. Source data are provided as a Source data file.

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