Fig. 3: Characterizations of assembly-driven azide–alkyne cycloaddition reaction.
From: Assembly drives regioselective azide-alkyne cycloaddition reaction

a HPLC traces of 2.5 mM Nap-FFK-Azi (green), 2.5 mM Nap-FFG-Alk (blue), 2.5 mM Nap-FFK-Tria-GFF-Nap (red), 2.5 mM Nap-FFK-Azi + Nap-FFG-Alk hydrogel at 4 °C for 7 d (orange), and 2.5 mM Nap-FFK-Azi + Nap-FFG-Alk in DMF at 4 °C for 7 d (black). Wavelength for detection: 254 nm. b TEM images of 2.5 mM Nap-FFK-Azi hydrogel, 2.5 mM Nap-FFG-Alk hydrogel, 2.5 mM Nap-FFK-Tria-GFF-Nap solution, and 2.5 mM Nap-FFK-Azi + Nap-FFG-Alk hydrogel after aging at 4 °C for 7 d. scale bar = 500 nm. c CD spectra of the samples in (b).