Fig. 8: Differential Inhibition of Pf and Pb parasites by MSO and PPT.
From: Distinct evolution of type I glutamine synthetase in Plasmodium and its species-specific requirement
a Effect of MSO on in vitro cultures of Pf in RPMI-gln medium. n = 4 independent experiments. b Giemsa-stained images of Pf parasites treated with MSO. c Effect of PPT on in vitro cultures of Pf in RPMI-gln medium. n = 3 independent experiments. d Giemsa-stained images of Pf parasites treated with PPT. e Effect of MSO and PPT on in vitro single-cycle cultures of Pb maintained in RPMI-gln medium. Giemsa-stained images of MSO and PPT-treated Pb schizonts are provided for 1 mM concentration. A growth assessment was carried out based on 3H-hypoxanthine uptake and verified by Giemsa-stained smears. n = 3 independent experiments. f Effect of MSO and PPT on in vivo Pb growth in Balb/c mice. Pb infections were initiated by injecting 105 parasites intraperitoneally on day 0. Mice were treated with the respective doses of MSO and PPT for four consecutive days starting from day 4. n = 3 different mice. Giemsa-stained images of MSO and PPT-treated Pb parasites are provided for 20 mg/kg treatment. Images for Giemsa-stained parasites were captured using 100x objective. Scale bar = 5 μM. For a, c, e, and f the data represent mean ± SD. Source data are provided as a Source Data file.
