Fig. 3: Sustained MSC-EV delivery by MN-MSC patch treatment alleviates neuroinflammation triggered by SCI. | Nature Communications

Fig. 3: Sustained MSC-EV delivery by MN-MSC patch treatment alleviates neuroinflammation triggered by SCI.

From: Porous microneedle patch with sustained delivery of extracellular vesicles mitigates severe spinal cord injury

Fig. 3

a Schematic illustration of MN-MSC patch implantation on the injury site of the spinal cord. b In vivo distribution of exosomes (DiI labeled, red) in the injured spinal cord tissues after MN-EV patch implantation, blue (DAPI). Scale bar: 1 mm. The injury region of the spinal cord is marked with yellow dotted lines. c Heatmaps of exosome distribution in the injured spinal cord tissues after MN-EV patch implantation; red: the highest numbers of exosomes, blue: the lowest, and white: background. The injury region of the spinal cord is marked with yellow dotted lines. b1, b2, c1, c2) Enlarged images to show the details. Scale bar: 100 μm. d HE staining of the injured spinal cord with MN-MSC patch implantation; scale bar: 1 mm. The experiment was repeated 3 times independently with similar results. The experiment was repeated 3 times independently with similar results. e Representative images of immunohistochemical staining for MN-MSC patches on the injured spinal cord on day 7 after SCI. Green (GFAP), blue (DAPI), red (GAPDH), scale bar: 1 mm. The experiment was repeated 3 times independently with similar results. f High-magnification images of the boxed area, scale bar: 50 µm. The experiment was repeated 3 times independently with similar results.

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