Fig. 2: Structural analysis of the Cu²⁺-containing complexes.

a SEM and b AFM images of the self-assembled Fmoc-K/GMP/Cu²⁺ complex. Inset is the cross-section analysis of the sample. [GMP]: 10 mM. [Fmoc-K]: 5 mM. [Cu²⁺]: 5 μM. n = 3 independent experiments. c Fluorescence emission spectra of the GMP/Cu²⁺, Fmoc-K/Cu²⁺ and Fmoc-K/GMP/Cu²⁺ complexes. [GMP]: 10 mM. [Fmoc-K]: 5 mM. [Cu²⁺] = 5 μM. d Experimental (solid) and simulated (dotted) EPR spectra of Fmoc-K/GMP/Cu²⁺, Fmoc-K/Cu²⁺ and GMP/Cu²⁺. [GMP]: 10 mM. [Fmoc-K]: 5 mM. [Cu²⁺] = 100 μM. e I₁/I₃ values of the mixtures of pyrene and Cu²⁺-containing complexes. [Pyrene] = 5 μM, [Cu²⁺] = 5 μM. [GMP] = 10 mM, [Fmoc-K] = 5 mM, [K] = 5 mM, [Boc-K] = 5 mM, [Cbz-K] = 5 mM. f K-edge X-ray absorption spectra and Fourier transforms of the k³-weighted EXAFS modulations for Fmoc-K/GMP/Cu²⁺ (red), Fmoc-K/Cu²⁺ (blue) and GMP/Cu²⁺(green). [GMP]: 10 mM. [Fmoc-K]: 5 mM. [Cu²⁺] = 200 μM. g Three-pulse ESEEM spectrum at 3436 G of Fmoc-K/GMP/Cu²⁺. τ = 200 ns. k = 1.81, η = 0.43. [Cu²⁺] = 250 μM. [Fmoc-K] = 5 mM, [GMP] = 10 mM.